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Louisiana Department of Health & Hospitals | Kathy Kliebert, Secretary

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Isolation, Confirmation and Enumeration of Vibrio species from Food or Enviromental Samples

PHL Location

Central Laboratory

CPT Code

N/A

Synonyms

 

Brief Description of Test

Isolation, confirmation and MPN determination of Vibrio species from food or environmental sample associated with outbreaks.

Possible Results

MPN of Vibrio species

Reference Range

N/A

Specimen Type

Food, Enviromental

Specimen Container(s):

Original packaging if available.  Rigid plastic containers, preferred sterile, provided in Rapid Response Kit.

Minimum volume accepted:

50 Grams

Collection Instructions

Collect food sample associated with food borne outbreak.  If food specimen has a lot number, collect 2 to 3 unopened food items with the same lot number.

Storage and Transport Instructions

Food sample must be stored and shipped in same condition received from submitter.   If specimen is received frozen (0°C or below), the food must be stored frozen (0°C or below), and shipped with dry ice or enough ice packs to keep frozen (0°C or below), during transit.  If specimen is received refrigerated (2-8°C), the food must be stored refrigerated (2-8°C), and enough ice packs to keep refrigerated (2-8°C), during transit.  Do not ship in wet ice, use ice packs.  Food sample must be shipped overnight to laboratory.

Causes for Rejection

Food sample was not stored or shipped in same condition received from submitter.  Specimen is received above 0°C instead of frozen (0°C and below), the food was not stored frozen (0°C or below), and shipped without dry ice or enough ice packs to keep frozen (0°C or below), during transit.  Specimen was received above refrigerated temperature (2-8°C), the food was not stored refrigerated (2-8°C), and food was shipped with not enough ice packs to keep refrigerated (2-8°C), during transit.  Food sample shipped in wet ice.  Food sample was not shipped overnight to laboratory.

Limitations of the Procedure

N/A

Interfering Substances

N/A

References

FDA BAM Online-Manual

Additional Information

Notify General Bacteriology for collection and storage instructions at (504) 219-4649.

Collect 75-100 Grams of food or environmental material

Use Lab Form 47 and supply the following information:

  • Food or Enviromental Source Specimen
  • Brand and Manufacturer if applicable
  • Lot number and Expiration date if applicable
  • Date and Time of Collection
  • Name of food borne outbreak associated with food or environmental sample
  • Test Requested ( Vibrio species Culture)
  • Submitter Information