Isolation and Confirmation of Vibrio cholerae from Food
|
PHL Location |
Central Laboratory |
|
CPT Code |
N/A |
|
Synonyms |
N/A |
|
Brief Description of Test |
Isolation and confirmation of Vibrio cholerae from food sample associated with food borne outbreaks. Serological testing performed on Vibrio cholerae that is isolated from the food sample. Molecular Laboratory performs toxin testing on Vibrio cholerae isolated. Futher biotyping performed by CDC on Vibrio cholerae isolates. |
|
Possible Results |
Vibrio cholerae 01 Inaba Toxigenic, Vibrio cholerae 01 Inaba Nontoxigenic, Vibrio cholerae 01 Ojawa Toxigenic, Vibrio cholerae 01 Ojawa nontoxigenic, Vibrio cholerae non 01 Toxigenic, Vibrio cholerae non 01 nontoxigenic. |
|
Reference Range |
N/A |
|
Specimen Type |
Food |
|
Specimen Container(s): |
Original packaging if available. Rigid plastic containers, preferred sterile, provided in Rapid Response Kit. |
|
Minimum volume accepted: |
25 Grams |
|
Collection Instructions |
Collect food sample associated with food borne outbreak. If food specimen has a lot number, collect 2 to 3 unopened food items with the same lot number. |
|
Storage and Transport Instructions |
Food sample must be stored and shipped in same condition received from submitter. If specimen is received frozen (0°C or below), the food must be stored frozen (0°C or below), and shipped with dry ice or enough ice packs to keep frozen (0°C or below), during transit. If specimen is received refrigerated (2-8°C), the food must be stored refrigerated (2-8°C), and enough ice packs to keep refrigerated (2-8°C), during transit. Do not ship in wet ice, use ice packs. Food sample must be shipped overnight to laboratory. |
|
Causes for Rejection |
Food sample was not stored or shipped in same condition received from submitter. Specimen is received above 0°C instead of frozen (0°C and below), the food was not stored frozen (0°C or below), and shipped without dry ice or enough ice packs to keep frozen (0°C or below), during transit. Specimen was received above refrigerated temperature (2-8°C), the food was not stored refrigerated (2-8°C), and food was shipped with not enough ice packs to keep refrigerated (2-8°C), during transit. Food sample shipped in wet ice. Food sample was not shipped overnight to laboratory. |
|
Limitations of the Procedure |
N/A |
|
Interfering Substances |
N/A |
|
References |
FDA BAM Online-Manual |
|
Additional Information |
Notify General Bacteriology for collection and storage instructions at (504) 219-4649. Collect 50-75 Grams of food material Use Lab Form 47 and supply the following information:
|
