Enterococci in Water
Fluorogenic Substrate Method for the Detection and Quantitation of Enterococci in Marine Water
PHL Location Central Laboratory, Lake Charles Laboratory, Shreveport Laboratory CPT Code N/A Synonyms Enterolert Test, Enterolert Most Probable Number Technique Brief Description of Test After incubation the diluted sample with reagent added is examined for fluorescence in the wells of the IDEXX Enterolert Quanti-tray / 2000. A table is referenced to find the Most Probable Number (MPN) of Enterococci present in the sample. Possible Results MPN / 100ml (colony forming units / 100ml) Reference Range < 10 to > 2005 Specimen Type Beach water Specimen Container(s): 250 ml polycarbonate sample bottle Minimum volume accepted: 200 ml Collection Instructions Samples should be collected by personnel trained in proper collection technique according to the Louisiana BEACH Program Sample Collection Standard Operating Procedures. Storage and Transport Instructions Samples should be placed on ice to ensure a temperature from 1-4ºC and delivered to the testing laboratory AS SOON AS POSSIBLE since sample analysis must begin within 6 hours after collection. Causes for Rejection Limitations of the Procedure Samples must be delivered to the lab within 6 hours of collection. The initiation of sample analysis must begin within 2 hours of receipt within the laboratory. The sample must be read within 24 to 28 hours. The lack of fluorescence after 28 hours is a valid negative test, but fluorescence after 28 hours is an invalid positive test result. Interfering Substances N/A References
