Isolation and Confirmation of Corynebacterium diphtheriae from a Clinical Specimen
Collect clinical specimen prior to initiation of antibiotic therapy for greatest chance of isolation of pathogen. Material for culture should be obtained with one of the following types of swab: Dacron, cotton or nasopharyngeal swab (NP). The swab must be placed on Loeffler's transport medium immediately after collection. If membranes are present and can be removed, swab from beneath the membrane. Refrigerate at 2-8°C after collection.
Storage and Transport Instructions
Specimens can be maintained at refrigerated temperatures (2°C to 8°C to protect viability. Do not freeze or ship frozen. Ship specimen overnight to Central Laboratory as soon as possible. The specimen should be received in the State Lab no later than 3 days from the original collection date.
Causes for Rejection
Limitations for the Procedure
The colleciton of a clinical specimen should occur prior to the initiation of antibiotic therapy for recovery of suspected microorganism. Colocintes of Corynebacterium diphtheriae maybe overgrown or inhibited by other members of resident flora.
**Submission of a clinical specimen requires prior authorization from the Louisiana OPH Infectious Disease Epidemtiology Program for submission to Central Lab.**
Use Lab Form 93 and supply the following information: