Louisiana Department of Health & Hospitals | Kathy Kliebert, Secretary

Statewide Initiatives

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Confirmation of a Pure Isolate of Bordetella pertussis/Bordetella parapertussis

Bordetella pertussis/Bordetella parapertussis

  • Laboratory Location – Central Laboratory
  • CPT Code –87077
  • Synonyms –Pertussis, Whooping Cough
  • Test Description - Confirmatory test for Bordetella pertussis/ Bordetella parapertussis

Possible Results

  • Bordetella pertussis/Bordetella parapertussis Confirmation

Reference Range

  • N/A

Specimen Requirements

  • Specimen Type - Any actively growing, pure culture of Bordetella pertussis/Bordetella parapertussis from a nasopharyngeal swab. 
  • Specimen Container(s) - Swap of actively growing culture sent in transport media that will support the growth of Bordetella pertussis/Bordetella parapertussis. Suggested treansport media:Regan-Lowe

Storage and Transport Instructions

Specimens can be maintained at ambient temperatures or refrigerated temperatures (2°C to 37°C) to protest viability. Do not freeze or ship frozen. The specimen should be received in the State Lab as soon as possible after the original collection date to allow for prompt epidemiological investigation.

Causes for Rejection

  • Leaking or broken container
  • Media is past the expiration date
  • The Lab form 93 missing required information
  • No lab slip received with specimen

Limitations for the Procedure

Culture submitted must be pure and viable

Interfering Substances

  • N/A


  • Manual of Clinical Microbiology 8th edition

Additional Information

Use Lab Form 93 and supply the following information:

  • Patient's Last Name and First Name
  • Patient's Gender
  • Patient's Date of Birth
  • Date and Time of Collection
  • Specimen Source
  • Test Requested (Bordetella pertussis/Bordetella parapertussis)
  • Submitter Information

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