Hemoglobinopathies, Adult
Hemoglobinopathies
- Laboratory Location – Central Laboratory
- CPT Code – 83021
- Synonyms –Hemoglobinopathies; SS; SC; SE; Sickle Cell Diease
- Test Description - The Separation and Identiifaction of Hemoglobin Variants by Isoelectrofocusing Electrophoresis
Possible Results
- AA: AC: AD: AE: AS: A+Barts: AV; CC; CD; CE; CS; AC+Barts; CC+Barts; CD+Barts; CE+Barts; CS+Barts; DD; DE; DS; AD+Barts; DD+Barts; DE+Barts; DS+Barts; EE; ES; AE+Barts; EE+Barts; ES+Barts; SS; AS+Barts; SS+Barts; AV+Barts; VV
Reference Range
Specimen Requirements
- Specimen Type - Dried Blood Spot on Filter Paper; Whole Blood
- Specimen Container(s) - Whatman 903 Filter Paper; Blood Collection Tubes with anticoagulants EDTA or HEPARIN
- Minumum accepted volume - one dried blood spot; one tube of whole blood
Specimen Collection Instructions
Blood collected via finger stick and blotted on filter paper, or Blood collected by venipuncture in EDTA or HEPARIN blood collection tubes
Storage and Transport Instructions
Storage of Blood Spot samples after collection: 2-30°C for up to 14 days. Whole Blood collected in EDTA or HEPARIN store at 2-8°C for up to 48 hours. Samples transported by state courier.
Causes for Rejection
- Insufficient blood on filter paper
- Expired filter paper form
- Short draw in blood collection tube or expired blood collection tube
- Whole blood collected in EDTA or HEPARIN > 48 hours old
Limitations for the Procedure
Isoelectric focusing separates hemoglobin variants with pI's that differ by 0.01 pH units of greater. Variants with the same pI migrate together. Positive identifcation of any specific variant may require actual sequencing of the amino acids in the globin chains. Electrophoresis of old samples may yield "aging bands" due to decomposition and/or oxidation of the hemoglobin molecule.
Interfering Substances
References
- Perkin Elmer's Resolve Hemoglobin Kit package insert
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