Specimen Collection Instructions
Blood collected via finger stick and blotted on filter paper, or Blood collected by venipuncture in EDTA or HEPARIN blood collection tubes
Storage and Transport Instructions
Storage of Blood Spot samples after collection: 2-30°C for up to 14 days. Whole Blood collected in EDTA or HEPARIN store at 2-8°C for up to 48 hours. Samples transported by state courier.
Causes for Rejection
Limitations for the Procedure
Isoelectric focusing separates hemoglobin variants with pI's that differ by 0.01 pH units of greater. Variants with the same pI migrate together. Positive identifcation of any specific variant may require actual sequencing of the amino acids in the globin chains. Electrophoresis of old samples may yield "aging bands" due to decomposition and/or oxidation of the hemoglobin molecule.