Hepatitis B Surface Antigen EIA
Blood should be collected in a plastic, sterile STD Program approved collection tube. Please follow the manufacturer's instructions on clot time and centrifuge speed/time requirements.
The Virology Lab 96 form must be filled out completely. The following information must be included on the form:
Specimens should be shipped refrigerated (2-8ºC) or at ambient temperatures for up to 7 days. For longer storage, specimens must be frozen at -20ºC or colder. Frozen specimens must be shipped on dry ice and received at a temperature of -20ºC or colder.
A designation of reactive for HBsAg must not be based on a single reactive test result. Additional testing, such as confirmatory testing, is required to establish the specificity of any specimen reactive by the screening procedure. Initially reactive specimens must be retested in duplicate to validate the initial test results. If, after repeat testing, the absorbance values of both duplicate specimens are less than the cutoff value, the original specimen may be considered non-repeatedly reactive and negative for HBsAg. If, after repeat testing, the absorbance value of either of the duplicates is greater than or equal to the cutoff value, the specimen must be considered repeatedly reactive. A HBsAg Confirmatory Assay is performed on repeatedly reactive specimens. The specimen can be considered positive for HBsAg only if the HBsAg can be neutralized by the confirmatory procedure.
