Feedback

Louisiana Department of Health & Hospitals | Kathy Kliebert, Secretary

PrintRSSShareTwitterFacebookWordPressYouTube
Statewide Initiatives



211 - Get Connected. Get Answers.

 

Hepatitis B Core IgG and IgM Antibody EIA

Hepatitis B Core IgG and IgM Antibody EIA

  • Laboratory Location – Central Laboratory
  • CPT Code – 86704
  • Synonyms – Total Antibodies to Hepatitis B Nucleocapsid Antigen (Core) EIA, Anti-HBc Total (IgG and IgM)
  • Test Description - The MONOLISA™ Anti-HBc EIA is an enzyme immunoassay intended for use in the qualitative detection of total antibodies to hepatitis B core antigen (anti-HBc) in human serum. Assay results may be used with other HBV serologic markers for the laboratory diagnosis of HBV disease associated with HBV infection.

Possible Results

  • Non-Reactive
  • Borderline
  • Reactive

Reference Range

  • Non-Reactive

Specimen Requirements

  • Specimen Type - Serum
  • Specimen Container(s) - red top tubes, Marble top tubes, polypropylene vials
  • Minumum accepted volume - 220 μL serum (does not allow for repeat testing)

Specimen Collection Instructions

Blood should be collected in a plastic, sterile STD Program approved collection tube. Please follow the manufacturer's instructions on clot time and centrifuge speed/time requirements.

The Virology Lab 96 form must be filled out completely. The following information must be included on the form:

  • Patient's Name
  • Patient's Date of Birth
  • Patient's Gender
  • Date and time of specimen collection
  • Test requested
  • Type of specimen collected
  • Submitter's name and address

Storage and Transport Instructions

Specimens should be shipped refrigerated (2-8ºC) within 48 hours of collection. For longer storage, specimens must be frozen at -20ºC or colder. Frozen specimens must be shipped on dry ice and received at a temperature of -20ºC or colder.

Causes for Rejection

  • expired collection tubes
  • incorrect specimen type
  • unspun samples
  • tubes that contain less than 90% of the total drawing capacity (QNS)
  • Improper storage or improper transport temperature requirements are also reasons for rejection

Limitations for the Procedure

For diagnostic purposes, results should be used in conjunction with patient history and other hepatitis markers for diagnosis of acute and chronic infection. A non-reactive test result does not exclude the possibility of exposure to hepatitis B virus. Results obtained with the MONOLISA™ Anti-HBc EIA assay may not be used interchangeably with values obtained with different manufacturers' Anti-HBc assay methods. Results from immunosuppressed patients should be interpreted with caution. A reactive anti-HBc result does not exclude co-infection by another hepatitis virus. The performance of the MONOLISA™ Anti-HBc EIA has not been established with cord blood, neonatal specimens, cadaver specimens or body fluids other than serum or plasma, such as saliva, urine, amniotic, or pleural fluids.

Interfering Substances

  • Heterophilic antibodies

References

  • BioRad MONOLISA™ Anti-HBc EIA package insert
  • EVOLISTM Operator Manual

 

Back to A to Z Test Listing