Hepatitis B Surface Antibody EIA
Blood should be collected in a plastic, sterile STD Program approved collection tube. Please follow the manufacturer's instructions on clot time and centrifuge speed/time requirements.
The Virology Lab 96 form must be filled out completely. The following information must be included on the form:
Speciments should be shipped refrigerated (2-8ºC) within 7 days of collection. For longer storage, specimens must be frozen at -20ºC or colder. Frozen specimens must be shipped on dry ice and received at a temperature of -20ºC or colder.
For diagnostic purposes, results should be used in conjunction with patient history and other hepatitis markers for diagnosis of acute and chronic infection. A non-reactive test result does not exclude the possibility of exposure to hepatitis B virus. Results obtained with the MONOLISA™ Anti-HBs EIA assay may not be used interchangeably with values obtained with different manufacturers' anti-HBs assay methods. Results from immuosuppressed patients should be interpreted with caution. This assay does not differentiate between a vaccine-induced immune response and an immune response induced by infection with HBV. To determine if the anti-HBs response is due to vaccine or HBV infection, a total anti-HBc assay may be performed. Performance characteristics have not been established for therapeutic monitoring. A reactive anti-HBs result does not exclude co-infection by another hepatitis virus. Individuals that have received blood component therapy (e.g., whole blood, plasma, immune globulin administration) during the previous 3 to 6 months may have a false reactive anti-HBs result due to passive transfer of anti-HBs. The performance of the MONOLISA™ ANti-HBs EIA has not been establised with cord blood, neonatal specimens, cadaver specimens, heat-inactivated specimens, or body fluids other than serum or plasma, such as saliva, urine, amniotic, or pleural fluids.
