Hepatitis A IgG and IgM Antibody EIA
Blood should be collected in a plastic, sterile STD Program approved collection tube. Please follow the manufacturer's instructions on clot time and centrifuge speed/time requirements.
The Virology Lab 96 form must be filled out completely. The following information must be included on the form:
Specimens should be shipped refrigerated (2-8ºC) within 48 hours of collection. For longer storage, specimens must be frozen at -20ºC or colder. Frozen specimens must be shipped on dry ice and received at a temperature of -20ºC or colder.
Diagnosis of an infectious disease should not be established on the basis of a single test result. Any diagnosis should take into consideration the patient's clinical history and symptoms, as well as other laboratory data. The assay calibrator is equivalent to 20 mIU/mL standardized to the WHO 2nd Reference Standard for Anti-hepatitis Immunoglobin. However, assay results cannot be considered quantitative and no clinical claims for immunity can be determined from the cutoff. A reactive anti-HAV result does not exclude co-infection by another hepatitis virus. The non-reactive result does not exclude the possibility of infection with hepatitis A virus. Levels of Anti-HAV may be below the cutoff in early infection. The performancce of the MONOLISA™ Anti-HAV IgM EIA has not been established with immunosuppressed or immunocompromised patients, cord blood, neonatal specimens, cadaveric specimens, heat-inactivated specimens, or body fluids other than serum or plasma, such as saliva, urine, amniotic, or pleural fluids. The performance of the MONOLISA™ Anti-HAV EIA detects both anti-HAV IgG and IgM antibodies. However, assays detecting total antibodies are known to be more sensitive for anti-IgG than IgM.
